Institutional Animal Care and Use Committee


​Animal research that falls under restricted Human Biomedical Research (rHBR)

The Fourth Schedule of the Human Biomedical Research Act (HBRA) regulates the conduct of restricted Human Biomedical Research (rHBR) in Singapore. If your animal research involves any of the following, you are required to declare this in your IACUC application: ​

(a) the following types of human-animal combination embryos:

​(i) cytoplasmic hybrid embryos;
(ii) human-animal combination embryos created by the incorporation of human stem cells (including induced pluripotent stem cells);
(iii) human-animal combination embryos created in-vitro by using —​

(A) human gametes and animal gametes; or​
​(B) one human pronucleus and one animal pronucleus​;

(b) the introduction of human stem cells (including induced pluripotent stem cells) into a prenatal animal foetus or animal embryo;

(c) the introduction of human pluripotent stem cells (including induced pluripotent stem cells) into a living postnatal animal;

(d) the introduction of human stem cells (including induced pluripotent stem cells) or human neural cells into the brain of a living postnatal animal; or

(e) any entity created as a result of any process referred to in sub paragraphs (b), (c) and (d).

In addition to NTU IACUC approval, you are required to obtain NTU IRB and MOH approval prior to commencement of research. ​

Projects not requiring NTU-IACUC approval

Research or teaching protocols involving the use of animals at NTU require NTU-IACUC approval before work on these projects can commence. There are however, certain categories of projects that do not require prior NTU-IACUC approval and these are listed here for reference. If you are unsure about the approval requirement for your project, please contact the IACUC Secretariat

  1. Those that involve commercially obtained animal tissues. If animal tissue samples are obtained from research entities, the PI must provide evidence of an approved IACUC protocol in place for the use of animals from which tissues were harvested.

  2. Those that involve the use of animal cells.

  3. Those involving the use of invertebrates such as snails, lobsters, starfish and insects.

  4. Those that involve the use of zebrafish embryos that are less than 7d.p.f. Embryos less than 7 d.p.f. are not able to feed upon hatching, are sustained by nutrients from the yolk and lack sensory and motor functions required for pain perception.
    All embryos after 7 d.p.f. and fish involved in the production of zebrafish embryos must be covered by an IACUC approved protocol.

  5. Those involving the use of fertilised eggs. PIs of such projects should however, email the IACUC Secretariat a written summary of the project for verification by the IACUC. After the IACUC has reviewed and approved the work, the PI may commence the project. The written summary should include

a.    Project title and objective(s).
b.    Declaration of what will be done to the embryonic tissues.
c.     Whether the eggs are obtained from a commercial hatchery or are purpose-bred.
d.    Measures that are in place to ensure that the fertilised eggs do not hatch.
e.    How the embryonic tissue / eggs will be disposed of at the end of experiments.