Academic Profile

Academic Profile

Assoc Prof Francesc Xavier Roca Castella

Associate Professor, School of Biological Sciences

Email: xroca@ntu.edu.sg
Phone: +65 65927561
Office: SBS 02N 18
Assoc Prof Francesc Xavier Roca Castella

Biography
During my PhD I studied the alternative splicing pathways of CD44 in human breast cells, showing that one of these pathways is de-regulated in ductal carcinomas. I then performed my post-doctoral research in Adrian R. Krainer Lab at Cold Spring Harbor Laboratory, New York (USA). There I investigated the basic mechanisms of 5' splice-site selection in humans using a combination of molecular, genomic and computational approaches. I also functionally characterized splicing mutations causing various genetic diseases, such as metabolic, neurological and muscular disorders. My major finding is the unexpected flexibility in the recognition of 5' splice sites by the U1 small nuclear RNA, which challenged a long-standing dogma and holds important implications for disease-causing splicing mutations and alternative splicing.
Research Interests
My research focuses on the study of the mechanisms of pre-messenger RNA splicing in human cells. Splicing is the process by which introns are excised and exons are joined together, and is essential for the correct expression of genes. The relevance of splicing in the big picture is further illustrated by these two facts: (1) a large fraction (~50%) of point mutations causing human hereditary disease and cancer affect splicing, and other syndromes are caused by alterations of splicing factors; (2) alternative splicing affects >90% of genes in the human genome, is critical to explain the complexity of the human transcriptome and proteome, and contributes to many processes at a cellular and organismal level. The molecular and phenotypic consequences of many disease-causing mutations at splicing elements are not predictable at present, and the mechanisms of alternative splicing and their impact on other biological processes are still largely unknown. These limitations stress the need to further understand the basic mechanisms of splicing. In my lab I address such type of questions by using a combination of computational, molecular and cell biology methods. The two long-term goals of my research are to improve the molecular diagnosis of splicing mutations causing human genetic disease and to elucidate the role of alternative splicing events in their biological contexts. In the future I also hope to identify new therapeutic targets, and/or to develop RNA-based therapies for human diseases.
Current Projects
  • Alternative splicing events and regulators important for human myeloid immunity
  • Characterization of the splicing functions of the two pseudouridines in U1 small nuclear RNA
  • Develop and apply four novel experimental tools to study the Xist RNA and human iPS cells
  • Development of Therapeutic Approaches to Overcome Drug Resistance Conferred by a Novel Bim Deletion Polymorphism in Chronic Myelogenous Leukemia and EGRF-Mutated Non-Small-Cell Lung Cancer
  • Dissecting X chromosome counting and reactivation by genetic screens
  • Functional characterization of the CPEB translation regulators in human monocytes and macrophages
  • Global analysis of alternative splicing upon differentiation of human monocytes
  • Implications of Splicing Mechanisms in Genetic Diseases and the Immune System Via CD46
  • Mechanisms of Splicing and their Implication in Human Genetic Disease
  • Structural and Functional Characterization of Antiviral Protein Variants Generated via Intronic Polyadenylation
Selected Publications
  • Juan WC, Roca X, Ong ST. (2014). Identification of cis-Acting Elements and Splicing Factors Involved in the Regulation of BIM Pre-mRNA Splicing. PLoS ONE, 9(4), e95210.
  • Roca X, Krainer AR, Eperon IC. (2013). Pick one, but be quick: 5' splice sites and the problems of too many choices. Genes & Development, 27(2), 129-144.
  • Roca X, Karginov FV. (2012). RNA biology in a test tube--an overview of in vitro systems/assays. Wiley Interdisciplinary Reviews in RNA, 3(4), 509-527.
  • Roca X, Akerman M, Gaus H, Berdeja A, Bennett CF, Krainer AR. (2012). Widespread recognition of 5' splice sites by noncanonical base-pairing to U1 snRNA involving bulged nucleotides. Genes & Development, 26(10), 1098-1109.
  • Kubota T, Roca X, Kimura T, Kokunai Y, Nishino I, Sakoda S, Krainer AR, and Takahashi MP. (2011). A mutation in a rare type of intron in a sodium-channel gene results in aberrant splicing and causes myotonia. Human Mutation, 32, 1-10.

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