Academic Profile

Academic Profile

Asst Prof Shashi Bhushan

Assistant Professor, School of Biological Sciences

Email: sbhushan@ntu.edu.sg
Phone: +65 65923673
Office: SBS 02N 01
Asst Prof Shashi Bhushan

Biography
Professional Experience

Junior Group Leader, DFG Research Center for Experimental Biomedicine, University of Wuerzburg, Germany
Postdoctoral Studies, Gene Center, LMU Munich, Germany
Research Interests
We are interested in determining 3D structures of macromolecular protein complexes to understand their function and mechanism. We employ Cryo-Electron Microscopy with single particle reconstruction as our main tool for structural determination. Our laboratory is equipped with advanced Electron Microscopes and high speed computing clusters for routine single particle reconstruction work. We are mainly interested in following research areas:


Protein translocation across cellular membranes
Co-translational protein folding
Protein secretion
Translational stalling and regulation
Current Projects
  • Biochemical and structural investigations of the mycobacterial MmpL3 transporter
  • Structural investigations of YidC mediated membrane protein insertion
  • Structural investigations of the organellar ribosomes of malaria parasite
Selected Publications
  • Wong CF, Shin J, Manimekalai MSS, Saw WG, Zhan Y, Bhushan S, Kumar A, Ragunathan P, Grüber G. (2017). AhpC of the mycobacterial antioxidant defense system and its interaction with its reducing partner Thioredoxin-C. Scientific Reports, 7, 5159.
  • Kumar V, Ero R, Ahmed T, Goh KJ, Zhan Y, Bhushan S, and Gao YG. (2016). The Structure of Elongation Factor 4 (EF4/LepA) in GTP Form Bound to the Ribosome. Journal of Biological Chemistry, 291(Cover Image July), 12943-50.
  • Singh D., Sielaff H., Sundararaman L., Bhushan S., Grüber G. (2015). The stimulating role of subunit F in ATPase activity inside the A1-complex of the Methanosarcina mazei Gö1 A1AO ATP synthase. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1857, 177-187.
  • Saha A, IChakraborty I, Kraft C, Bhushan S and Ghosh S. (2013). Microtubule nucleation from a functionalised SiO2 EM grid. RSC Advances, 3, 7688–7691.
  • Winzer A, Kraft C, Bhushan S, Stepanenko V and Tessmer I. (2012). Correcting for AFM tip induced topography convolutions in protein-DNA samples. Ultramicroscopy, 121, 08-15.

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