Note: Cell-free samples are not considered human tissues.
|Examples considered to be substantial manipulation||Examples not considered to be substantial manipulation|
|Culture expansion of cells from tissues, cell lines created from tissues, formalinfixed paraffin-embedded tissue, enzymatic treatment to dissociate cell cell contact, breaking open to cells to recover subcellular components. ||Cutting, grinding, shaping, centrifugation, sterilization, low-level irradiation, cell separation, concentration/purification, filtering, lyophilisation, freezing, cryopreservation, vitrification.|
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