|Our work is focused on structural and functional aspects of membrane proteins, leading to understanding of their function and to development of drugs that will modify their activity. |
One type of protein we explore is the aquaporins, the 'water channels' of the cell. Aquaporins selectively transport water and this makes them very useful for biotechnological applications such as water purification. In a medical context, aquaporins are found in most human cells and are involved in a vast array of pathological conditions, but no drugs are available for inhibition or activation. Therefore discovery of aquaporin inhibitors is one of our main interests.
Another system we study are small membrane proteins from enveloped viruses, which tend to oligomerize and form channels of different selectivities. Some examples are M2 from influenza A virus, p7 from hepatitis C virus, SH from respiratory syncytial virus and coronavirus envelope proteins. No structural data exists for the full length version of these proteins despite their small size (typically less than 100 amino acids). Further, their flexibility likely contributes to their multiple reported functions.
- Aquaporin Based Biomimetic Membranes For Water Reuse and Desalination
- Membrane protein sciences - Tools for rational discovery of novel therapeutics and diagnostics targeting integral membrane proteins
- Optimisation of a Mimic Transmembrane Peptide Delivery System, Consisting of a Chimeric Bacterial-Hairpin-Like Construct, for Specific Labelling of Membrane Proteins. In Vitro & in Vivo Appln to Structure & Function of the SARS CoV E Protein
- Synthesis, structure and inhibition of p7, a viroporin found in the hepatitis C virus.
- Siok-Wan Gan, Edward Tan, Xin Lin, Dejie Yu, Juejin Wang, Gregory Ming-Yeong Tan, Ardcharaporn Vararattanavech, Chiew Ying Yeo, Cin Huang Soon, Tuck Wah Soong, Konstantin Pervushin and Jaume Torres. (2012). The small hydrophobic protein of the human respiratory syncytial virus forms pentameric ion channels. Journal of Biological Chemistry, .
- Siok Wan Gan, Ardcharaporn Vararattanavech, Nurhuda Nordin, Said Eshaghi, Jaume Torres. (2011). A COST EFFECTIVE METHOD FOR SIMULTANEOUS HOMO-OLIGOMERIC SIZE DETERMINATION AND MONODISPERSITY CONDITIONS FOR MEMBRANE PROTEINS. Analytical Biochemistry, .
- A. Bhunia, H. Mohanram, P.N. Domadia, J. Torres, and S. Bhattacharjya. (2009). Designed beta-boomerang antiendotoxic and antimicrobial peptides: Structures and activities in lipopolysaccharide. Journal of Biological Chemistry, .
- K. Pervushin, E. Tan, K. Parthasarathy, X. Lin, F.L. Jiang, D. Yu, A. Vararatannavech, T.W. Soong, D.X. Liu and J. Torres. (2009). Structure and inhibition of the SARS Coronavirus Envelope Protein Ion Channel. PLoS Pathogens, .
- Krupakar Parthasarathy, Lifang Ng, Xin Lin, Ding Xiang Liu, Konstantin Pervushin, Xiandi Gong and Jaume Torres. (2008). Structural flexibility of the pentameric SARS coronavirus envelope protein ion channel. Biophysical Journal, 95, L39-L41.