|Membrane organization and mechanism of protein sorting in the cyanobacterium Synechocystis sp. PCC 6803.|
Cyanobacteria are unique among eubacteria in exhibiting an organized subcellular compartmentation in the form of a highly differentiated membrane system with the internal thylakoid membrane in addition to the outer and plasma membranes. Thus there are six different compartments in cyanobacteria, three membranes and three soluble compartments, periplasm, cytoplasm and lumen, leading to a complicated system for transport and sorting of proteins into the different membranes and compartments. Isolation of pure membranes of each type has been possible by a unique method developed in my group, which exploits two different attributes of membranes, surface properties and density. By shotgun and gel-based proteomics of isolated plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803 a large number of membrane proteins were identified. Proteins localized uniquely in each membrane, as well as overlapping proteins, can be used as a platform for describing a model for cellular membrane organization and protein sorting into the different cellular compartments. Uniquely localized integral membrane proteins were analyzed by multivariate sequence analysis in order to trace potential differences in sequence properties important for insertion to the correct membrane. The analysis showed that information in the whole protein or in the C-terminal region was required for class separation, indicating a post-translocon sorting mechanism. The results can be discussed in terms of membrane organization. Two different opinions prevail concerning the organization of plasma and thylakoid membranes. One is that the two membranes are continuous, making the periplasm and lumen a common compartment, the second that the membranes are completely separated.
Recent publications within the project
Pisareva, T., Kwon, J., Oh, J., Kim, S., Ge, C., Wieslander, Å., Choi, J-S and Norling, B. ”A model for membrane organization and protein sorting in the cyanobacterium Synechocystis sp. PCC 6803; proteomics and multivariate sequence analyses” manuscript
Zhang, LF., Yang, HM., Cui, SX., Hu, J., Wang, J., Kuang, T., Norling, B., and Huang, F. (2009) ”Proteomic Analysis of Plasma Membranes of Cyanobacterium Synechocystis sp. Strain PCC 6803 in Response to High pH Stress”. J Proteome Res. 8, 2892-2902
Pisareva , T., Shumskaya , M., Maddalo, G., Ilag, L., and Norling B. (2007) “Proteomics of Synechocystis sp. PCC 6803: Identification of novel integral plasma membrane proteins” FEBS J. 274, 791-804
Rajalahti, T., Huang, F., Edman, M., Pisareva, T., Rosén, M., Sjöström, M., Wieslander, Å., and Norling, B. (2007) ”Proteins in different Synechocystis compartments have distinguishing N-terminal sequence features. A combined proteomics and multivariate data analysis” J. Proteom. Res. 6, 2420-2434
Fulda, S., Mikkat, S., Huang, F., Huckauf, J., Martin K., Norling, B., and Hagemann , M. (2006) ”Proteome analysis of salt stress response in the cyanobacterium Synechocystis sp. strain PCC 6803” Proteomics 6, 2733-2745
Srivastava, R., Battchikova, N., Norling, B., Aro, EM. (2006) ” Plasma membrane of Synechocystis PCC 6803: a heterogeneous distribution of membrane proteins” Arch. Microbiol. 185, 238-243
Huang, F., Fulda, S., Hagemann, M. and Norling, B. (2006) ”Proteomic screening of salt-stress induced proteins in plasma membranes of Synechocystis sp. PCC 6803”, Proteomics 6, 910-920
Srivastava, R., Pisareva, T., and Norling , B. (2005) ”Proteomic studies of the thylakoid membrane of Synechocystis sp.PCC 6803”, Proteomics 5, 49015-4916
- Lifang Zhang*, Tiago Toscano Selao*, Tatiana Pisareva, Jingru Qian, Siu Kwan Sze, Inger Carlberg and Birgitta Norling. (2013). Deletion of Synechocystis sp. PCC 6803 Leader Peptidase LepB1 Affects Photosynthetic Complexes and Respiration. Molecular and Cellular Proteomics, 12(5), 1192-203.
- Pisareva T, Kwon J, Oh J, Kim S, Ge C, Wieslander Å , Choi JS, Norling B. (2011). Model for membrane organization and protein sorting in the cyanobacterium Synechocystis sp. PCC 6803 inferred from proteomics and multivariate sequence analyses. Journal of Proteome Research, 10, 3617-3631.
- Kwon J, Oh J, Park C, Cho K, Kim SI, Kim S, Lee S, Bhak J, Norling B, Choi JS. (2010). Systematic cyanobacterial membrane proteome analysis by combining acid hydrolysis and digestive enzymes with nano-liquid chromatography-Fourier transform mass spectrometry. Journal of Chromatography A, 1217, 285-293.