Academic Profile

Academic Profile

Asst Prof Wu Bin

Nanyang Assistant Professor, School of Biological Sciences

Email: wubin@ntu.edu.sg
Phone: +65 69082207
Office: EMB 06 06
Asst Prof Wu Bin

Biography
Education

School of Biological Sciences, Nanyang Technological University, Singapore
Ph.D. in Biochemistry, Biophysics and Structural Biology, 2006.07-2010.02
B.Sc. in Biological Sciences, 2002.07-2006.06


Research Experience

Charles A. King Research Fellow, Harvard Medical School, 2010-present
Advisor: Sun Hur
Dissected the molecular mechanisms for viral RNA recognition and signaling by RIG-I/MDA5 and MAVS, an important innate immune signaling pathway related to multiple diseases. Investigated viral dsRNA recognition process of MDA5 by using techniques including biochemistry, molecular biology, X-ray crystallography and electron microscopy (Wu et al, Cell, 2013). This work is exclusively highlighted in Nature Review Immunology 2013. Established an in vitro reconstituted assay system to study MAVS signaling, which was applied to study related innate immune receptors and lead to the identification of RNA dependent filament formation as an important signaling mechanism in innate immunity (Peisley & Wu et al, Mol Cell, 2013) & (Peisley, Wu, et al, Nature, 2014). In addition, I also solved the MAVS filament structure (Wu et al, Mol Cell, 2014).

Graduate Student, Nanyang Technological University, 2006-2010
Advisor: Curt A. Davey
Using a combination of biochemical and biophysical tools, I characterized the molecular basis of therapeutic effects of Pt based anti-cancer drugs. Demonstrated how these drugs inhibited the dynamics of the basic functional units of our genome, the nucleosomes (Wu et al, Nature Chemical Biology, 2008). This work is also highlighted in Nature 2008. Additional work on biochemical and structural studies of nucleosomes with a variety of DNA sequences or modified DNA directly demonstrated the evidence of DNA sequence dependent nucleosome.
Research Interests
Auto-immune diseases are often caused by mis-regulated innate immune responses. The recent discoveries of a collection of innate immune receptors and their downstream adaptors provided us with the unprecedented opportunities to investigate and tackle the related diseases. However, at the moment, the effort of developing related therapies is largely prohibited by a lack of knowledge of molecular information of the key components of the signaling pathways.

Based on my previous success and experience in characterizing MDA5 and RIG-I, two cellular viral RNA sensors in innate immunity, I propose to investigate their downstream adaptor protein, MAVS, and other related innate immune signaling pathways. I plan to employ a combination of biophysical, biochemical and cellular methodologies to achieve my research goals.

I encourage undergraduate and graduate students sending in enquiries about opportunities performing cutting-edge research in molecular immunology. There are also Project Officer positions available.

please email WUBIN@ntu.edu.sg for further information.
Current Projects
  • C-di-GMP-mediated host-pathogen interactome at molecular/systems biology level
  • Harnessing the Innate Immunity, Biochemical and Structural Studies of RLR and NLR Signaling Pathways
  • Hybrid high resolution structure determination of helical filaments using cryo-EM and solid state NMR spectroscopy
  • Quicklase - Feasibility studies of antibody and cellular engineering in immunotherapy
  • Structure Determination of NLRP6: Towards defining its unique role in innate immunity, microbiota, and homeostasis of the gut
  • Systematic Engineering of OaAEP1 as Dynamic Enzymatic tools
Selected Publications
  • MUSTAFA FAZLI, Morten Rybtke, Elisabeth Steiner, Elisabeth Weidel, JENS BERTHELSEN, Julie Groizeleau, Wu Bin, Boo Zhao Zhi, Zhang Yaming, Volkhard Kaever, MICHAEL GIVSKOV, Rolf Hartmann, Leo Eberl, TIM TOLKERNIELSEN. (2017). Regulation of Burkholderia cenocepacia biofilm formation byRpoN and the c-di-GMP effector BerB. MicrobiologyOpen, .
  • Wu B, Peisley A, Tetrault D, Li Z, Egelman EH, Magor K, Thomas Walz T, Penczek P, Hur S. (2014). Molecular imprinting as a signal activation mechanism of the viral RNA sensor RIG-I. Molecular Cell, 55(4), 511-23.
  • Peisley A, Wu B, Hui X, Chen ZJ and Hur S. (2014). Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Nature, 509, 110-114.
  • Peisley A*, Wu B*, Yao H, Walz T and Hur S. (2013). RIG-I forms signaling-competent filaments in an ATP-dependent and ubiquitin-independent manner. Molecular Cell, 51(5), 573-83.
  • Wu B, Hur S. (2013). Viral counterattack against the host innate immune system. Cell Research, 23(6), 735-736.

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