Academic Profile

Academic Profile

Prof Gerhard Gruber

Professor, School of Biological Sciences

Email: ggrueber@ntu.edu.sg
Prof Gerhard Gruber

Biography
Date of 1st Appointment: 11th of August 2005
Present Appointment: Associate Professor

● Academic Qualification
2002 Ph.D. (habil.) in Biochemistry and Molecular Biology, University of the Saarland, Germany
2001 Ph.D. (habil.) in Biochemistry, University of Osnabrück, Germany
1995 Ph.D. (rer. nat.) in Biochemistry, Johannes Gutenberg-University Mainz, Germany
1992 Diploma Degree, Johannes Gutenberg-University Mainz, Germany

● Awards
1995 - 1996, Postdoc-Fellowship of the German academical exchange service (DAAD)
1997 - 1999, Fellowship of the Human Frontiers Science Programs (HFSP)
2000 - 2001, Habilitation-Fellow of the German Research Foundation (DFG)

● Administrative involvements
1. Deputy Head of Division of Structural and Computational Biology, School of Biological Sciences,
Nanyang Technological University
2. Member of the Steering committee of the Singapore Bioimaging Consortium (SBIC)
Research Interests
Research Activities
The research group on Structure and function of molecular motors of A/Prof. G. Gruber in the Division of Structural and Computational Biology, SBS, NTU, is recognized for their expertise in determining the relationships between the structure and function of the so-called A1AO ATP synthases, V1VO ATPases, F1FO ATP synthases and AAA-ATPases. These enzymes are proposed to be the smallest biological motor proteins (nano-motor proteins). In order to get inside into the structure of these multi-subunit membrane complexes techniques like single particle analysis of electron micrographs, solution X-ray scattering, X-ray crystallography and NMR-spectroscopy are used. The functional and dynamical processes inside these enzymes are studied by fluorescence spectroscopy (e.g. FRET, FCS, intrinsic fluorescence spectroscopy) and biochemical approaches.
Current Projects
  • An Integrated Framework to Study the Dynamics of BiologicalStructures
  • Biological nanomotors for drug delivery and energy production
  • Defining the structural traits of subunits D and E of the biological energy producer A1A0 ATPsynthase
  • Functional and mechanistic understanding of the biological motor protein F1F0 ? ATP synthase due to structural approaches
  • Functional and structural characterization of functional domains of the Reticulocyte Binding Proteins of Plasmodium during merozoite invasion
  • Insights into the mechanisms and structure of the key coupling subunits 􀄰􀀃and 􀈖􀀃of the Mycobacterium tuberculosis F1FO ATP synthase, and their potential as novel TB drug target
  • RESEARCH PACKAGE(3 YEARS)
  • START-UP GRANT
  • Structrual and biochemical characterization of the nucleotide-binding subunits A and B and the A-H interaction inside the archaea type A1A0ATPsynthase
  • Structural and mechanistic understanding of HAMLET, a tumouricidal protein
  • Target Based discovery of next generation pyrazinamide
  • Target based discovery of next generation pyrazinamide
  • Targeting Oxidative Phosphorylation for the Rational Development of Sterilising Drug Combination for Drug-resistant Tuberculosis
  • Touch the Molecule
  • Understanding the Transition State and Assembly Formation of the Escherichia coli Alkyl Hyperoxide Reductase complex, an Essential Enzyme to alleviate Oxidative Stress
  • Understanding the mechanisms and structure of the Mycobacterium tuberculosis bifunctional (p)ppGpp synthase/hydrolase, RelMtb
  • Understanding the structure and assembly of subunits E,F,G and a of nature's most versatile pump, V-ATPase
  • Understanding the structure and mechanisms of the Alkylhydroperoxide Reductase subunits C and F, a key enzyme ensemble for Antioxidant defence in vancomycin-resistant Enterococcus faecalis
Selected Publications
  • Saw, W.-G., Wong, C.-F., Dick, T., Grüber, G. (2020). Overexpression, purification, enzymatic and microscopic characterization of recombinant mycobacterial F-ATP synthase. Biochemical and Biophysical Research Communications, 522(2), 374-380.
  • Saw, W.-G., Wu, M.-L, Ragunathan, P., Biuković, G, Lau, A.-M., Shin, J., Harikishore, A., Cheung, C.-Y., Hards, K., Sarathy, J. P., Bates, R. W., Cook, G. M. C., Dick, T., and Grüber, G. (2019). Disrupting coupling within mycobacterial F-ATPsynthase subunit ε causes dysregulated energy production and cell wall biosynthesis. Scientific Reports, 9, 16759.
  • Kamariah, N., Ragunathan, P., Shin, J., Saw, W.-G., Wong, C.F., Dick, T., and Grüber, G. (2019). Unique structural and mechanistic properties of mycobacterial F-ATP synthases: Implications for drug design. Progress in Biophysics & Molecular Biology, in press.
  • Saw, W.G., Pan, A., Manimekalai, M.S.S., Grüber, A., and Grüber, G. (2019). Structure and flexibility of Non-structural proteins 3 and -5 of Dengue- and Zika viruses in solution. Progress in Biophysics & Molecular Biology, 143, 67-77.
  • Saw, W.G., Chan, K., Vasudevan, S.G. and Grüber, G. (2019). Zika virus non-structural protein 5 residue 681 is critical for dimer formation and enzymatic activity. FEBS Letters, 593(12), 1272-1291.

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