|Assoc Prof Ajai Vyas||This section pertains to current research directions of my team.
We are interested in diverse research questions at the intersection of neuroendocrinology; parasitology and behavioral ecology. We take pride in braiding mechanistic view of biology with its mooring in the evolution and ecological conditions.
We are broadly interested in backwash effects of death on the processes of the life. Finite nature of the life creates a variety of trade-offs in individuals. We are interested in how these trade-offs reflect in brain and hormones. We use perturbation models in our approach. These models include manipulation of host behavior by coevolving parasites; breakdown of learning and memory due to aging; and effects of predator presence on prey physiology. These diverse approaches are united by our vision of placing form and function of neuroendocrine system within a framework of biological evolution.
We have a healthy disrespect for artificial separation between fundamental and clinical biology. Our work relates to both fundamental understanding of biological processes (e.g., trade-off between reproduction and defense in brain or non-consumptive effects of predation) and issues more closer to human condition (e.g., optogenetic modulation of memory in dementia models or sexually transmitted protozoan infections).
|Dr Alessandra Bonanni||Biosensor technologies
Biosensors are analytical tools which combine a biorecognition agent which provides selectivity and a transducer that confers sensitivity and can convert the biorecognition event into a measurable electronic signal. The advantage of using biosensor over traditional techniques is represented by their low costs, small dimensions, portability, and fast response. The different areas for potential biosensor applications are mainly medical diagnosis, environmental monitoring and food analysis.
DNA analysis is of extreme importance to solve problems of different nature such as investigation on genetic diseases, detection of food and water contamination by microorganism, studies on breeding origins or tissue matching, and solution of forensic issues. The techniques for genome identification are nowadays mainly based on DNA sequencing by using fluorescent labels and optical detectors. They require a few days for the analysis to be completed and the costs are prohibitive to the general public. Demand has never been greater for innovative technologies which can provide fast, inexpensive and reliable genome information.
The focus of my research is on the development of analytical tools (biosensors) for the rapid, reliable and low cost identification of DNA sequences. In order to achieve that, different electrochemical techniques are used for the detection of the analytical signal. The developed biosensors are based on various platforms (such as gold, carbon and silicon) and different nanomaterials (i.e. gold nanoparticles, graphene nanoplatelets and several quantum dots) are employed for the amplification of the obtained signal and the improvement of detection limit.
The final step of this work is the application of the developed genosensor to real sample analysis. Once this second part is successfully accomplished, the analytical tool could be eventually integrated into a DNA amplification process, resulting in a portable device for point-of-care diagnostic tests and for very sensitive detection of SNPs correlated to different diseases.
This field includes research in the two following areas:
- Application of DNA genosensors to identify of DNA sequences correlated to bacteria involved in food contamination (i.e. Salmonella spp, Listeria monocytogenes, Escherichia coli)
- Development of electrochemical biosensors for the detection of antioxidant capacity of food and beverages
|Assoc Prof Alfred Tok Iing Yoong||1) Carbon-based Field-Effect Transistor Sensors
The biosensors market, which is currently at USD 9.9 billion, is expected to reach USD 18.9 billion in 2019 (GIA Report, 2014) propelled by the growing population and health issues. Our group capitalizes on this emergent market and researches on disposable and low-cost sensor suitable for real-time sensing in field conditions. Our group focuses on sensors for biological and gas detection applications.
2) Synthesis of Nanostructured Materials using Atomic Layer Deposition (ALD)
Atomic layer deposition (ALD) has evolved to be a unique tool for nanotechnology with atomic level control of the depositions, 3D conformity and homogeneity. Film depositions can be realized for complex non-planar topographies for a wide range of applications such as energy conversion and storage, nanoparticle catalysts, nanostructures for drug delivery, gas separations, sensing, and photonic applications. Our group focuses on ALD materials for solar cell, hydrogen generation and smart window applications.
3) Hard & Tough Materials for Ballistic Protection Application
The next generation of military vehicular and soldier system requires light-weight materials with high strength-to-weight ratio. Our research focuses on the synthesis and densification of nanostructured materials & desired composite architecture to significantly raise the ballistic protection capability. The B-C-N-O group of compounds are potential candidates to form novel materials for ballistic protection application as they inherent the unique properties from both boron nitride and boron carbide which are known for their light weight, high hardness, low friction coefficient and high wear resistance. Prof Tok leads a team of collaborators in armour material research ranging from high temperature synthesis of novel superhard materials and consolidation by state-of-the-art Spark Plasma Sintering to advanced characterisation techniques such as depth of penetration test using Two-Stage Light-Gas Gun.
4) Institute for Sports Research
Our group is involved in the Institute for Sports Research, working on the damping property of midsoles which is based on carbon nanotube (CNT). CNT’s high aspect ratios (length/diameter) is particularly desirable for mechanical reinforcement, and it is found that the vertical aligned (VA)CNTs perform well in damping, to dissipate the energy absorbed under compression (Figure 7). Our present job is to tune the damping property of VACNT by adjusting the length, diameter and area density etc. parameters and try to reinforce the polymer with VACNT to fabricate midsole material with better cushion property.
In accordance with the objectives of the Energy Thrust Program of the NRF-CREATE Project, our group is focused on the design and synthesis of highly functional nanomaterials, which enables energy harvesting and conservation. Recently, novel graphene oxide synthesized nanoballs and nanoflowers were synthesized. These exhibit potentials for supercapacitors and energy applications. In general, these activities results in above 50 publications, 17 patent applications and projects discussions with companies regarding commercialization possibilities.
|Assoc Prof Ali Miserez||Structural properties of biological materials from the macro-scale to the nano-scale
Multi-scale structural and mechanical properties of biological materials, including biominerals.
Elastomeric and structural properties of bioelastomers
Protein chemistry of sclerotized hard-tissues from marine organisms, such as Cephalopod
Single-molecular force spectroscopy of structural and elastic proteins
Underwater adhesion mechanisms of adhesive proteins
RNA-sequencing and proteomics of extra-cellular biological materials
Advanced Metal/Ceramic composites
Experimental Fracture Mechanics
|Asst Prof Amartya Sanyal||The main focus of our research is to understand 3D genome organization inside the nucleus and its impact on transcriptional regulatory code during mammalian development, differentiation and disease. Please visit Sanyal Lab webpage (http://www.ntu.edu.sg/home/asanyal).
Human genome is organized in highly complex conformations inside the nucleus. How this three-dimensional organization of chromatin affects gene regulation is largely unknown. Genome-wide annotations of genes and functional regulatory elements do not give an insight into which regulatory elements control any given gene. Long-range looping interactions between gene promoters and distal genomic elements such as enhancers are known to be important for regulation of transcription. The advent of Chromosome Conformation Capture (3C)-based techniques and its high-throughput adaptations has made it possible to detect spatial proximity and high-resolution chromatin interactions between genomic elements.
We are particularly interested in understanding how non-coding sequence variants identified by genome-wide association studies (GWAS) contribute to human disease risk and pathogenesis. In the past decade, genome-wide scans of SNPs (single nucleotide polymorphisms) in populations have identified many genomic loci associated with the predisposition to disease. The observed associations are possibly driven by linkage disequilibrium with the disease-associated region in vicinity. However, >90% GWAS SNPs do not map to coding regions suggesting these variants may, in fact, affect gene regulatory mechanism and involved in controlling the expression of distal target genes, the identity of which remain unknown. Connecting the GWAS SNPs to their target genes would aid in understanding genotype-phenotype relationships in disease and in designing effective treatment and therapeutics.
In our lab, we intend using high-throughput genomic methods, genome-editing and imaging techniques in combination with bioinformatics and computational approaches to understand structure-function relationship of chromatin. Overall, we are trying to decipher the regulatory mechanisms of cell- and tissue-specific gene expression in relation to 3D chromatin architecture, epigenetic mechanisms (chromatin modifications) and binding of trans-acting factors to understand various biological processes in normal and disease conditions.
|Assoc Prof Arindam Basu||Low-power Reconfigurable Mixed-signal design, Neural recording systems, Computational neuroscience, Nonlinear dynamics, Smart sensors for hearing-aids/ultrasound etc, Neuromorphic VLSI
|Prof Atul N. Parikh||Membrane biophysics
biologically inspired materials
synthetic chemical biology
|Asst Prof Ayumu Tashiro||Our lab studies the function of hippocampus circuitry through an interdisciplinary approach combining virus-mediated genetic manipulation, optogenetics and unit recording techniques in behaving rodents. The hippocampus is well known for its functions in memory formation. We are interested in how neuronal circuits in the hippocampus mediate these functions. We particularly focus on two distinct phenomena occurring in the adult hippocampus.
The first is adult neurogenesis (generation of new neurons), which occurs exclusively in a hippocampal subregion called the dentate gyrus and a few other area outside the hippocampus. We are interested in 1) how neural processing/activity in the dentate gyrus determines birth of new neurons and their subsequent functional integration into existing circuits and 2) how those new neurons integrated into circuits contribute to the hippocampal functions.
The second focus is the cellular mechanism of place-cell activity. Place cells are electrophysiologically-defined cell types found in multiple subregions of the hippocampus. When animals explore environments, place cells fire at specific locations in the environments, which suggests that place-cell activity mediates spatial memory processing in the hippocampus. We investigate cellular mechanism underlying properties of place-cell activity using virus-mediated local genetic manipulations.
Our goal is to understand physiological mechanisms in the hippocampus and to provide basic information which is critical to treat and cure brain disorders including dementia, neurodegenerative disease and depression.
|Prof Bertil Andersson||Photosynthesis research, biological membranes, protein and membrane purification, light stress in plants.
In addition, author of a number of articles devoted to popular sciences and science policy.
|Prof Bo Gunnar Liedberg||The research interests of Prof. Bo Liedberg can be divided into three main areas
Surface Chemistry and Self Assembled Monolayers
This part of the research concerns fundamental studies of adsorbates and ultrathin molecular architectures, like Self-Assembled Monolayers (SAMs), on solid supports. The group was very early in studying self-assembly of substituted alkylthiols on gold substrates. A key activity has been to study temperature driven phenomena occurring in such assemblies as well as in adsorbed layers on top such SAMs. Oligo(ethylene glycol) and oligosaccharide SAMs have attracted considerable attention, both experimentally and theoretically, because of their structural characteristics and advantageous properties in contact with biofluids. Another area concerns interfacial water and ice. Temperature programmed studies have been undertaken to improve the understanding of the nucleation and microscopic wetting behavior of water/ice. The complexity of the SAMs has increased over the years and we are today focusing on architectures based on SAMs bearing multivalent chelator heads, helix-loop-helix polypeptides and receptor functions.
Bioinspired and Biomimetic Nanoscience
This research concerns the development of nanoscale architectures fabricated using either top-down or bottom-up protocols (or a combination of both). We are, for example, developing plasmonic arrays based on 100 nm gold nano dots on silicon and glass surface for amplification of optical fluorescence signals, so-called metal enhanced fluorescence (MEF). We are also developing composite materials based on a combination of de novo designed peptide scaffolds, planar surfaces and nanoparticles of controlled size and shape. A novel concept based on peptide folding has been used for controlled assembly of gold nanoparticles. The group is also involved in the development of Dip Pen Nanolithography (DPN) for patterning of surfaces on the 30-100 nm length scale. This work is performed jointly with a previous student of the group who nowadays is setting up a nanolaboratory at the Institute of Physics, Vilnius. We are also involved in several EC projects where different types of micro- and nanoscale patterning tools are employed for production of coatings for biofouling, sensing and biomedical applications.
Optical Biosensors, micro- and nanoarrays
The group has a long experience in developing optical transducers for biosensing application. We were the first to demonstrate the use of surface plasmon resonance for studies of bioaffinity interactions at surfaces, a technology that today form the backbone in SPR/Biacore instruments developed for biospecific interaction analysis (BIA). We are today using it in combination with ellipsometric interrogation and imaging optics for microarraying, and in combination with nanoparticle for studies optical enhancement phenomena. This includes, for example, microarray chips for protein multiplexing. The group is also working on the development of generic biochips for studies of ligand-receptor binding. Besides working on microarray fabrication for protein detection and analysis we are also developing biochips for the safety and security area.
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